Journal:

Article Title: GalR1, but not GalR2 or GalR3, levels are regulated by galanin signaling in the locus coeruleus through a cyclic AMP-dependent mechanism

doi: 10.1111/j.1471-4159.2005.03105.x

Figure Lengend Snippet: GalR1 is upregulated in a cyclic AMP-dependent manner in the LC-like cell line, Cath.a. A) Cath.a cells were stimulated for 5 min with 5 μM forskolin to induce cyclic AMP production and PKA activation. Activation of the cyclic AMP/PKA signaling pathway was analyzed by western analysis. Representative western blots are shown for CREB (Ser133) phosphorylation, total CREB and GAPDH (loading control). An increase in CREB (Ser133) phosphorylation indicates forskolin-induced activation of the cyclic AMP/PKA pathway. B) Cath.a cells were stimulated with 5 μM forskolin for 7h to determine cyclic AMP-dependent changes in galanin receptor protein expression. Representative western blots are shown for GalR1, GalR2, GalR3 and GAPDH. C) Galanin receptor protein levels were quantified using NIH Image software and significance was determined by ANOVA. * p < 0.001.

Article Snippet: Blots were washed 3 times for 5 min each, then incubated for 30 min at room temp in anti-GAPDH monoclonal primary antibody (Advanced ImmunoChemical Inc., Long Beach, CA) diluted to 1:2000 in 5% BSA/TBS.

Techniques: Activation Assay, Western Blot, Phospho-proteomics, Control, Expressing, Software

Journal: Stem Cell Research & Therapy

Article Title: Differential expression profiles of long noncoding RNAs and mRNAs in human bone marrow mesenchymal stem cells after exposure to a high dosage of dexamethasone

doi: 10.1186/s13287-020-02040-8

Figure Lengend Snippet: The sequences of primers for qRT-PCR

Article Snippet: The primary antibody for rabbit anti-human GAPDH and all secondary antibodies were purchased from Elabscience (Shanghai, China).

Techniques: Sequencing

Journal: Stem Cell Research & Therapy

Article Title: Differential expression profiles of long noncoding RNAs and mRNAs in human bone marrow mesenchymal stem cells after exposure to a high dosage of dexamethasone

doi: 10.1186/s13287-020-02040-8

Figure Lengend Snippet: Effect of Dex on the osteogenic and adipogenic differentiation of the hBMSCs. a ARS staining of the hBMSCs after treatment with various concentrations of Dex (10 −8 mol/L, 10 −7 mol/L, and 10 −6 mol/L) for 14 days. b The results of the western blotting analysis showing the protein expression levels of the osteogenic markers (BSPII and Runx-2) after treatment for 14 days, and c quantification of the integrated density of the target bands normalized to GAPDH levels. d The results of the qRT-PCR showing the mRNA expression of osteogenic markers (BSPII and Runx-2) after treatment for 14 days, and quantification through normalization to GAPDH levels. e ORO staining of the hBMSCs after treatment with various concentrations of Dex (10 −8 mol/L, 10 −7 mol/L, and 10 −6 mol/L) for 14 days. f The results of the western blotting analysis showing the protein expression levels of the adipogenic markers (PPAR-γ and CEBP-α) after treatment for 14 days, and g quantification of the integrated density of the target bands after normalization to GAPDH levels. h The results of the qRT-PCR showing the mRNA expression levels of adipogenic markers (PPAR-γ and CEBP-α) after treatment for 14 days, and the quantification through normalization to GAPDH levels. Note: all results are presented as mean ± standard deviation of three independent experiments. * P < 0.01 compared with the control group; # P < 0.01 between the two groups. hBMSCs, human bone marrow mesenchymal stem cells; Dex, dexamethasone; BSPII, bone sialoprotein II; Runx-2, runt-related transcription factor-2; PPAR-γ, peroxisome proliferator-activated receptor-γ; CEBP-α, CCAAT/enhancer-binding protein-α

Article Snippet: The primary antibody for rabbit anti-human GAPDH and all secondary antibodies were purchased from Elabscience (Shanghai, China).

Techniques: Staining, Western Blot, Expressing, Quantitative RT-PCR, Standard Deviation, Control, Binding Assay

Journal: Biochemistry and Biophysics Reports

Article Title: An appropriate loading control for western blot analysis in animal models of myocardial ischemic infarction

doi: 10.1016/j.bbrep.2017.09.001

Figure Lengend Snippet: Protein levels of β-tubulin, β-actin and GAPDH in the heart tissue from animal model of myocardial ischemia. (A, B) Western blot and quantitative analysis of β-tubulin, β-actin and GAPDH protein levels in heart tissue from Rhesus monkey model of early stages of myocardial ischemia (2 h after surgery, n = 2; 24 h after surgery, n = 2). (C, D) Western blot and quantitative analysis of β-tubulin, β-actin and GAPDH protein levels in heart tissue from Rhesus monkey model of myocardial infarction (4 months after surgery, n = 4). (E, F) Western blot and quantitative analysis of β-tubulin, β-actin and GAPDH protein levels in heart tissue from mouse model of different time point (1 d after surgery, n = 6; 4 d after surgery, n = 6; 7 d after surgery, n = 6). Sham: sham operated control (n = 8); IA: ischemic/ infarct area; RA: remote area. Blots were analyzed by densitometry using Fusion-Capt Software. Results are presented as means ± SD. Three independent experiments were run for each result. *: P < 0.05 versus sham operated control; #: P < 0.05.

Article Snippet: The blots were then incubated with primary antibodies anti-β-actin, GAPDH, and β-tubulin, (at 1:1000 dilution, ZSGB-BIO, China) overnight at 4 °C.

Techniques: Animal Model, Western Blot, Control, Software